Gadolinium in PDB 1b9y: Structural Analysis of Phosducin and Its Phosphorylation-Regulated Interaction with Transducin Beta-Gamma

The structure of Structural Analysis of Phosducin and Its Phosphorylation-Regulated Interaction with Transducin Beta-Gamma, PDB code: 1b9y was solved by R.Gaudet, P.B.Sigler, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 3.00
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	76.470, 89.920, 102.340, 90.00, 90.00, 90.00
R / Rfree (%)	21.3 / 26.6

The binding sites of Gadolinium atom in the Structural Analysis of Phosducin and Its Phosphorylation-Regulated Interaction with Transducin Beta-Gamma (pdb code 1b9y). This binding sites where shown within 5.0 Angstroms radius around Gadolinium atom.
In total 6 binding sites of Gadolinium where determined in the Structural Analysis of Phosducin and Its Phosphorylation-Regulated Interaction with Transducin Beta-Gamma, PDB code: 1b9y:
Jump to Gadolinium binding site number: 1; 2; 3; 4; 5; 6;

Gadolinium binding site 1 out of 6 in the Structural Analysis of Phosducin and Its Phosphorylation-Regulated Interaction with Transducin Beta-Gamma


Mono view


Stereo pair view

A full contact list of Gadolinium with other atoms in the Gd binding site number 1 of Structural Analysis of Phosducin and Its Phosphorylation-Regulated Interaction with Transducin Beta-Gamma within 5.0Å range: probe atom residue distance (Å) B Occ A:Gd341 b:64.5 occ:1.00 O A:GLN44 2.6 40.6 1.0 O C:HOH255 2.9 28.5 1.0 O A:HOH345 2.9 57.8 1.0 O A:HOH348 3.2 19.7 1.0 C A:GLN44 3.7 40.6 1.0 CA A:GLN44 4.5 40.6 1.0 N A:MET45 4.6 53.1 1.0 N A:GLN44 4.6 40.6 1.0 CB A:GLN44 4.6 61.4 1.0 OXT A:ASN340 4.6 73.7 1.0 CA A:MET45 4.6 53.1 1.0 O A:ASN340 4.7 58.9 1.0 OH C:TYR224 4.9 49.8 1.0

Gadolinium binding site 2 out of 6 in the Structural Analysis of Phosducin and Its Phosphorylation-Regulated Interaction with Transducin Beta-Gamma


Mono view


Stereo pair view

A full contact list of Gadolinium with other atoms in the Gd binding site number 2 of Structural Analysis of Phosducin and Its Phosphorylation-Regulated Interaction with Transducin Beta-Gamma within 5.0Å range: probe atom residue distance (Å) B Occ A:Gd342 b:0.9 occ:1.00 OD1 A:ASP228 2.5 81.5 1.0 O A:CYS204 3.0 58.6 1.0 CG A:ASP228 3.4 81.5 1.0 OD2 A:ASP228 3.6 81.5 1.0 C A:CYS204 4.1 58.6 1.0 CB A:CYS204 4.2 80.9 1.0 CA A:CYS204 4.6 58.6 1.0 CB A:ASP228 4.8 81.5 1.0 N A:ASP228 4.9 56.7 1.0

Gadolinium binding site 3 out of 6 in the Structural Analysis of Phosducin and Its Phosphorylation-Regulated Interaction with Transducin Beta-Gamma


Mono view


Stereo pair view

A full contact list of Gadolinium with other atoms in the Gd binding site number 3 of Structural Analysis of Phosducin and Its Phosphorylation-Regulated Interaction with Transducin Beta-Gamma within 5.0Å range: probe atom residue distance (Å) B Occ B:Gd101 b:63.5 occ:1.00 OD2 B:ASP542 2.5 74.5 1.0 O B:HOH1 2.6 56.9 1.0 OE2 B:GLU546 2.8 78.2 1.0 OE1 B:GLU546 2.8 78.2 1.0 O B:HOH18 3.1 80.0 1.0 CD B:GLU546 3.1 78.2 1.0 O B:HOH6 3.2 56.5 1.0 CG B:ASP542 3.6 74.5 1.0 GD B:GD104 4.1 66.8 1.0 OD1 B:ASP542 4.1 74.5 1.0 CG B:GLU546 4.4 78.2 1.0 NH1 B:ARG547 4.8 44.6 1.0 CB B:ASP542 4.9 74.5 1.0

Gadolinium binding site 4 out of 6 in the Structural Analysis of Phosducin and Its Phosphorylation-Regulated Interaction with Transducin Beta-Gamma


Mono view


Stereo pair view

A full contact list of Gadolinium with other atoms in the Gd binding site number 4 of Structural Analysis of Phosducin and Its Phosphorylation-Regulated Interaction with Transducin Beta-Gamma within 5.0Å range: probe atom residue distance (Å) B Occ B:Gd104 b:66.8 occ:1.00 O B:HOH10 2.5 52.3 1.0 O B:HOH19 2.7 64.9 1.0 OD1 B:ASP542 2.8 74.5 1.0 OE2 B:GLU546 3.0 78.2 1.0 O B:HOH15 3.2 70.2 1.0 CG B:ASP542 3.7 74.5 1.0 CD B:GLU546 3.7 78.2 1.0 OD2 B:ASP542 3.9 74.5 1.0 OE1 B:GLU546 3.9 78.2 1.0 GD B:GD101 4.1 63.5 1.0 CG B:GLU546 5.0 78.2 1.0

Gadolinium binding site 5 out of 6 in the Structural Analysis of Phosducin and Its Phosphorylation-Regulated Interaction with Transducin Beta-Gamma


Mono view


Stereo pair view

A full contact list of Gadolinium with other atoms in the Gd binding site number 5 of Structural Analysis of Phosducin and Its Phosphorylation-Regulated Interaction with Transducin Beta-Gamma within 5.0Å range: probe atom residue distance (Å) B Occ C:Gd247 b:64.3 occ:1.00 OE1 C:GLU218 2.5 63.0 1.0 O C:HOH253 2.7 58.1 1.0 O C:HOH250 2.7 44.3 1.0 O C:HOH249 2.7 63.0 1.0 OE2 C:GLU161 2.8 51.5 1.0 OE1 C:GLU161 2.9 51.5 1.0 OE2 C:GLU218 2.9 63.0 1.0 CD C:GLU218 3.1 63.0 1.0 CD C:GLU161 3.2 51.5 1.0 CG C:GLU218 4.6 63.0 1.0 CG C:GLU161 4.7 51.5 1.0 OH C:TYR162 4.8 33.9 1.0

Gadolinium binding site 6 out of 6 in the Structural Analysis of Phosducin and Its Phosphorylation-Regulated Interaction with Transducin Beta-Gamma


Mono view


Stereo pair view

A full contact list of Gadolinium with other atoms in the Gd binding site number 6 of Structural Analysis of Phosducin and Its Phosphorylation-Regulated Interaction with Transducin Beta-Gamma within 5.0Å range: probe atom residue distance (Å) B Occ C:Gd248 b:0.9 occ:1.00 OE2 C:GLU223 2.8 64.1 1.0 OE1 C:GLU223 3.1 64.1 1.0 OE1 B:GLU566 3.2 0.9 1.0 O C:HOH251 3.2 78.1 1.0 CD C:GLU223 3.3 64.1 1.0 O A:HOH347 3.3 77.5 1.0 O C:HOH254 3.4 47.9 1.0 OE2 B:GLU566 3.5 0.9 1.0 CD B:GLU566 3.7 0.9 1.0 O C:HOH252 4.5 52.6 1.0 NH2 A:ARG46 4.6 37.5 1.0 O A:THR47 4.8 42.1 1.0 CG C:GLU223 4.8 64.1 1.0

R.Gaudet, J.R.Savage, J.N.Mclaughlin, B.M.Willardson, P.B.Sigler. A Molecular Mechanism For the Phosphorylation-Dependent Regulation of Heterotrimeric G Proteins By Phosducin. Mol.Cell V. 3 649 1999.
ISSN: ISSN 1097-2765
PubMed: 10360181
DOI: 10.1016/S1097-2765(00)80358-5